Part one of term paper
SUMMARY Investigations of the integrity and transport characteristics of 2/4/A1 cells have been done in this report. The cell line was isolated from rat fetal intestinal epithelial cells and transfected with thermolabile SV40 large T antigen. These cells proliferated at 33 °C, but eliminated the antigen and ceased proliferating at a non-per ...

Part two of term paper
... microscopy confirmed the development of multilayers at 33°C, monolayers at 37°C and defects in the cell layer due to apoptosis at 39°C. Different immunostainings of ZO-1, E-cadherin and vinculin confirmed formation of tight and adherence junctions. Transepithelial resistance reached a plateau of 25-35 Ohm.cm2, which was similar to the small intestine. In transport studies 2/4/A1 cell line monolayers selectively restricted the permeation of hydrophilic permeability markers proportional to mol ...